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Where to find Tanshinones98%UV&Tanshinone IIA95HPLC from MDidea Group?Inhibition

From: derrida michael E-mail:derrida@vip.163.com  http://www.mdidea.com
Category: 商业信息
Date: 8/4/2003
Time: 5:55:18 PM
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Where to find Tanshinones98%UV&Tanshinone IIA95HPLC from MDidea Group?Inhibition of mast cell degranulation by tanshinones from the roots of Salvia miltiorrhiza?

Amanuensis&Symbol trace Calligrapher: Michael Derrida

famous processor of Tanshinones & Tanshinone IIA in high concentration.,JUST con tact w ith or check as following: http://www.mdidea.com/products/monomer/mono14.html

Tanshinones98%UV&Tanshinone IIA95HPLC(Origin:Radix Salviae Miltiorrhizae,Danshen ) ◇◇◇◇◇◇◇◇◇◇◇◇◇◇◇◇

Basic Data: [Chemical Name]:Tanshinones,Tanshinone IIA [Molecular Formula and Molecular Weight]：C19H18O3；294.33 [Botanical Source]:Przewalsk Sage Root; [Botanical Synoms]:Radix Salviae Przewalskii,Salvia Przewalskii Maxim.;Salvia miltiorrhiza;Salvia, danshen Basic Function: anti-inflammatory activitor;Mast cell degranulation Inhibitor,apoptosis induci ng activity;anticancer drugs,apoptosis-inducing activitor,Anti-aggregator，Vasodi latic，Anti-inflammatory，Antiseptic，Rheumatology/Anti-rheumatic，anti-cancer,

Where to find Tanshinones98%UV&Tanshinone IIA95HPLC from MDidea Group?Inhibition of mast cell degranulation by tanshinones from the roots of Salvia miltiorrhiza?

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Abstract:Tanshinone IIA is a diterpenoid naphthoquinone found in the traditio nal Chinese medicine Tanshen (Salvia sp). It inhibits AP-1 activity by suppressin g jun-fos-DNA complex formation IC50=0.22 µM). It displays anti-inflammator y activity and induces apoptosis in a variety of cell lines.

The activity-guided fractionation of the extract of the root of Salvia miltio rrhiza B. (Labiatae, Tanshen), led to the isolation of four active components res ponsible for the anti-allergic activity in vitro. Among them, 15,16-dihydrotanshi none-1 and cryptotanshinone demonstrated significant inhibition of the release of beta-hexosaminidase from cultured RBL-2H3 cells in a dose-dependent manner; the ICS, values were calculated as 16 and 36 mu M, respectively

Dosage, Toxicity,And Adverse Effects

The Pharmacopoeia of the People's Republic of China (20) indicates a recommen ded dosage of 9-15 grams per daily dose in decoction form. In a few instances, hi gher doses are administered, up to 20 grams per day, in the treatment of inflamma tory diseases, including viral hepatitis. According to English-Chinese Rare Chine se Materia Medica, up to 30-60 grams can be used in cases of angina and heat-type arthritis (21). The relatively high dosage of salvia, compared to most other Chi nese herbs (typical dosage recommendations are 3-9 grams for many herbs) may be a ttributed to the relatively low level of active constituents and their poor solub ility in water.

At the higher dosage levels, salvia may on rare occasions cause dry mouth, di zziness, lassitude, numbness, shortness of breath, and other symptoms that will u sually disappear spontaneously without interrupting the treatment. Rare Chinese M ateria Medica notes that salvia is not suitable for patients who have deficiency but not stasis, or deficiency accompanied by cold, or with tendency to bleed. How ever, most materia medica guides do not present these cautions. Salvia has very l ow acute toxicity, with an LD50 by injection of 40-80 g/kg (19).

It is recommended that salvia, or its preparations used for treatment of poor blood circulation, not be combined with coumadin (Warfarin), as there is a possi bility of increasing the anticoagulant effects (see: The interactions of herbs an d drugs). In a literature survey conducted through October 2000, three cases of i ncreased anticoagulant activity were reported in the literature in persons taking salvia along with Warfarin (25). Such effects may be rare and are likely to be d ose dependent, as the mechanism appears to be a simple additive effect of anticoa gulant activity of salvia along with that produced by Warfarin. Therefore, person s using coumadin should either avoid using salvia, or use it in relatively low do sage (not more than the equivalent of 6-9 grams per day in decoction) while payin g attention to blood coagulation tests that are routinely performed for persons t aking the drug.

Recently, the anti-tumour effect of tanshinones isolated from Salvia miltiorr hiza BUNGE was elucidated using human tumour cell lines. Since many anticancer dr ugs induce apoptosis of tumour cells, the apoptosis-inducing activity of the crud e extract of Salvia miltiorrhiza BUNGE was investigated. Human premyelocytic leuk emia cell line, HL60, was treated with various concentrations of the ether extrac t of Salvia miltiorrhiza BUNGE for 4 h, and it was found that it induced internuc leosomal DNA fragmentation, one of the biochemical hall mark of apoptosis, at the concentration above 10 μg/ml. UV radiation was used as a positive control for t he induction of apoptosis. Among the constituents of Salvia miltiorrhiza BUNGE, t anshinone IIA was focused on, because it is most abundant and structurally repres entative having a basic tanshinone skeleton. Using a similar phytochemical proced ure with the previous report, tanshinone IIA was successfully purified and the st ructure of it was clearly identified compared with the previous report. When HL60 cells were treated with various concentrations of tanshinone IIA, internucleosom al DNA fragmentation was induced by the concentrations as low as 1 μg/ml. In tim e course experiment in which 3 μg/ml of tanshinone IIA was treated, 180 bp ladde r was generated at 2 or 3 hours after the treatment. Microscopic observation show ed that the crude ether extract of Salvia miltiorrhiza BUNGE and purified tanshin one IIA both induced cellular morphological changes characteristic of apoptosis i ncluding membrane blebbing and apoptotic body formation. Flow cytometry analysis of tanshinone IIA-treated HL60 cells showed the increase of hypodiploid apoptotic cells and the decrease of the cells at G1 phase of cell cycle, suggesting a poss ibility that tanshinone IIA induced apoptosis occurs at G1 phase of cell cycle. P ARP is a nuclear enzyme which is involved in DNA repair process, and recently, it was found that 113 kDa PARP protein is cleaved into 89 and 24 kDa fragments by t he action of CPP32, a protease recently named as caspase-3.

Since the specific proteolytic cleavage of PARP is considered to be a biochem ical characteristic of apoptosis, the Western blotting experiment was carried out using the antibody against PARP. The results demonstarted that PARP is cleaved i nto 89 kDa fragment 4 hours after the addition of tanshinone IIA suggesting that caspase-3 was activated. To measure the caspase-3 activity directly and quantitat ively, Ac-DEVD-pNA was used, a specific colorimetric substrate of caspase-3. The results demonstrated that caspase-3 is activated during tanshinone IIA-induced ap optotic process. Recently, many papers reported that internucleosomal DNA fragmen tation is not essential in apoptotic cell death, and some necrotic cell death is accompanied by internucleosomal DNA fragmentation, suggesting the possibility tha t internucleosomal DNA fragmentation may be not enough as an indicator of apoptot ic cell death. It is, however, clear that the central mechanism of apoptosis is e volutionary conserved and caspase activation is an essential step in this complex apoptotic pathways. The results of the study, therefore, give more important evi dences that tanshinone IIA-induced cell death is apoptosis.

In this study the apoptosis inducing activity of tanshinone IIA, a main ingre dient of Salvia miltiorrhiza BUNGE, was examined. Other tanshinone components str ucturally related to tanshinone IIA such as cryptotanshinone and tanshinone I als o exist in the root of Salvia miltiorrhiza BUNGE, and the apoptosis-inducing acti vity of other tanshinones will be examined later especially focusing on caspase a ctivity which is central component of apoptotic pathway.

Basic Functions of Tanshinones:

Tanshinones as anti-inflammatory activitor:As reported by Gottlieb in 1997, t he management of osteoarthritis should include specific dietary and supplementati on practices, in addition to other natural treatments such as joint mobilization, manipulation, muscle therapy, acupuncture and exercise. In this regard glucosami ne sulfate has demonstrated the ability to halt joint cartilage destruction and h elp regenerate new cartilage in osteoarthritis cases. However, there is also subs tantial clinical and experimental evidence to suggest that the inflammatory aspec t of many forms of arthritis and joint inflammatory conditions can be treated eff ectively with the use of certain supplements, which demonstrate anti-inflammatory properties. In fact, small clinical trials indicate that many of these natural a gents provide similar efficacy as conventional anti-inflammatory drugs,

Tanshinones as Non Steroidal Anti-Inflammatory Drugs

Non Steroidal Anti-Inflammatory Drugs (NSAIDs) are medications which, as well as having pain-relieving (analgesic) effects, have the effect of reducing inflam mation when used over a period of time.

Tanshinones as NSAIDs can be used as simple pain killers (analgesics), but pa racetamol is usually preferable, as it is likely to have less unwanted effects, a nd costs less. They are most useful in conditions which cause inflammation. The a nti-inflammatory effects may take from a few days to three weeks to come on, so i t is worth persevering for a while before deciding that a NSAID is not going to h elp.

Tanshinones as NSAIDs are used as follows:

The commonest use of these drugs is for arthritis. Paracetamol is often adequ ate for osteoarthritis, but NSAIDs including Tanshinones are particularly useful in the inflammatory forms of arthritis (eg rheumatoid arthritis) and, sometimes, in the more severe forms of osteoarthritis. Back pain and sciatica. Ibuprofen has been clearly demonstrated to be helpful , and the other NSAIDs are also helpful. Sprains, strains, and rheumatism. Dental pain. Post-operative pain. Period pain (dysmenorrhoea) and heavy periods (menorrhagia). Pain from kidney stones (renal colic). To help reduce temperature in someone with a fever. Migraine. Other painful conditions, especially where there is inflammation.

Properties:

The NSAIDs work by affecting some chemicals in the body which cause inflammat ion, the prostaglandins. Unfortunately the same group of chemicals are involved i n the stomach, and so the NSAIDs tend to cause indigestion, and may even cause du odenal or stomach ulceration.

As a result of this side-effect they cannot be used in someone with a history of peptic ulcer, except in exceptional circumstances, under close medical superv ision. Also they would rarely be used and, if used, only with extra care, in some body with heartburn or indigestion.

In general, the more effective a NSAID is at reducing inflammation, the more likely it is to cause indigestion. Sometimes your doctor will prescribe them alon g with something to cut down the risk of ulceration. There is even one medication that contains both components together.

There have been recent advances, in that some NSAIDs are said to be more spec ific in dealing with inflammation and less likely to irritate the digestive (gast ro-intestinal) system, but nothing has yet overcome this problem altogether.

The drugs vary in strength and side effects. Usually, as with other medicatio ns, the more effective they are, the more side-effects they are likely to have. A spirin, which originated from willow bark, has been around for a long time and is in many people's medicine chests. This is an anti-inflammatory analgesic. Most N SAIDs also reduce the temperature in someone with a fever.

Of the newer medications in this group, the one in widest general use is Ibup rofen, which is available over the counter in many countries. There are a large n umber of other NSAIDs, most of which have to be obtained on prescription in the U K.

Tanshinones as Mast cell degranulation Inhibitor:Tanshinones could be act as best Mast cell degranulation Inhibitor, and helpe to make good anti cancer drugs and widely used more often and welcomed by most medicine developer.

apoptosis-inducing activitor,

Anti-aggregator，

Vasodilatic，

Antiseptic，Antiseptic Wound Healer:antiseptic herbs for quick healing of cut s, athlete's foot, ringworm and acne. Tanshinones could be Formulated with Golden seal root and rubbing alcohol and made into A great addition to your first aid ki t medicine.

Rheumatology/Anti-rheumatic，

Where to find Tanshinones98%UV&Tanshinone IIA95HPLC from MDidea Group?

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------------------------------------------ Scintific References: References: 1.Where to find Tanshinones98%UV&Tanshinone IIA95HPLC from MDidea Group?Inhi bition of mast cell degranulation by tanshinones from the roots of Salvia miltior rhiza? By Michael Derrida 2.What is danshen?Inhibition of mast cell degranulation by tanshinones from the roots of Salvia miltiorrhiza? By Michael Derrida ? 3.Where to find Tanshinones98%UV&Tanshinone IIA95HPLC from MDidea Group?Inhi bition of mast cell degranulation by tanshinones from the roots of Salvia miltior rhiza?

Amanuensis&Symbol trace Calligrapher: Michael Derrida

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======================================================= famous processor of Tanshinones & Tanshinone IIA in high concentration.,JUST con tact w ith or check as following: http://www.mdidea.com/products/monomer/mono14.html

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